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Biparatopic anti-GFP vNAR antibody, clones JP01&JP03 (iFluor® 488)
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Mxiture of two shark derived anti-GFP vNAR single domain antibodies (molecular weight: 14.4 kDa, 13.9 kDa), expressed in E. coli under conditions free from animal derived components
原料试剂 研发实验室
价格
¥1305.00-3653.10
品牌 Jotbody
地区 中国,香港特别行政区,新界
货号 JOT0204-1
产地 进口
选择规格
5 ug
50 ug
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Jotbody 捷博生物
深圳市捷博生物科技有限公司
深圳
营业执照已审核
Jotbody(捷博生物)成立于2020年,专注纳米抗体和外泌体领域,提供专业CRO服务及优质试剂产品。公司系香港城市大学孵化,由生物制药与生命科学工具领域的顶尖大学教授、资深企业家和海归博士共同创立,搭建先进纳米抗体发现平台、工程化外泌体平台和高性能试剂开发平台,研发创新试剂适配科研、分析检测及诊断多场景。作为国家高新技术企业,《福布斯亚洲》“2023年亚洲最值得关注100家企业”,服务已覆盖30余个国家和地区。
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产品规格 图文详情 技术文档
产品规格
品牌名称
Jotbody
货号
JOT0204-1
国产/进口
进口
规格
5 ug,50 ug
货源
新品
图文详情

Basic Information

SpecificitySpecies ReactivityCloneConjugate and TagsTested Applications
Green fluorescent protein (GFP)Species independentJP01&JP03iFluor® 488IF
PurificationProduct Buffer SolutionFormatReconstitutionAnalyte Description
Affinity chromatography purified via Ni-charged resinPBS, pH 7.4LyophilizedReconstitute the lyophilized protein with sterile deionized water or with 50% glycerol (ACS grade or better) to a stock solution of 0.5 mg/mL. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Avoid vigorous shaking or vortexing.Green fluorescent protein (GFP) is a protein composed of 238 amino acid residues(26.9kDa) derived from the Jellyfish Aequorea victoria, which emits green light (emission peak at 509nm) when excited by blue light (excitation peak at 395nm). GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. EGFP contains the doubleamino-acid substitutions Phe-64 to Leu and Ser-65 to Thr (previously published as GFPmutl; PMID: 8707053). In contrast to wiGFP, EGFP has a single, strong, redshifted excitation peak at 488 nm.

 

Parameters on Each Lot

Product ConcentrationPurityAffinity ConstantPositive ControlsRecommended
Dilutions
0.5 mg/mL by Nanodrop≥95% (4-12% gradient SDS-PAGE)KD of 20 pM by Biacore T200Positive IF detected in GFP-expressing HEK293 cellsIF 1:200-1:1000
技术文档
文件Datasheet for JOT0204-1.pdf
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