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收藏Basic Information
| Specificity | Species Reactivity | Clone | Conjugate and Tags | Tested Applications |
| Green fluorescent protein (GFP) | Species independent | JP01&JP03 | iFluor® 488 | IF |
| Purification | Product Buffer Solution | Format | Reconstitution | Analyte Description |
| Affinity chromatography purified via Ni-charged resin | PBS, pH 7.4 | Lyophilized | Reconstitute the lyophilized protein with sterile deionized water or with 50% glycerol (ACS grade or better) to a stock solution of 0.5 mg/mL. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Avoid vigorous shaking or vortexing. | Green fluorescent protein (GFP) is a protein composed of 238 amino acid residues(26.9kDa) derived from the Jellyfish Aequorea victoria, which emits green light (emission peak at 509nm) when excited by blue light (excitation peak at 395nm). GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. EGFP contains the doubleamino-acid substitutions Phe-64 to Leu and Ser-65 to Thr (previously published as GFPmutl; PMID: 8707053). In contrast to wiGFP, EGFP has a single, strong, redshifted excitation peak at 488 nm. |
Parameters on Each Lot
| Product Concentration | Purity | Affinity Constant | Positive Controls | Recommended Dilutions |
| 0.5 mg/mL by Nanodrop | ≥95% (4-12% gradient SDS-PAGE) | KD of 20 pM by Biacore T200 | Positive IF detected in GFP-expressing HEK293 cells | IF 1:200-1:1000 |
Datasheet for JOT0204-1.pdf