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| Source: |
Human HEK293 cell line, HEK293-derived human Noggin Fc chimera protein | ||||||
| Accession: | Q13253 | ||||||
| Purity: | >90%, by SDS-PAGE under reducing conditions. | ||||||
| Endotoxin Level: | <0.10 EU/μg of the protein by the LAL method. | ||||||
| Activity: | Recombinant human Noggin Fc chimera protein inhibits BMP2 inducted luciferase expression in the NIH-3T3-BRE, which is a BMP responsive reporter cell line. The luciferase expression was analyzed using the Bright-Lite Luciferase Assay System (Vazyme Biotech). The ED50 for this effect is 0.100-0.500 µg/mL in the presence of 10ng/mL of recombinant human BMP-2 (Proteintech# HY-P7006). | ||||||
| Organoids Culture Test: | Pass | ||||||
| Structure: | Disulfide-linked homodimer. | ||||||
| Predicted Molecular Weight | 49.4 kDa (monomer). | ||||||
| SDS-PAGE | 58-62 kDa, reducing conditions. | ||||||
| Sterile: | 0.22μm sterile filtration. | ||||||
| Product Form: | Lyophilized powder. | ||||||
| Shipping & Storage: | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below: Ø To the date of expiration, -20°C to -80°C as supplied. Ø 3 months, -20°C to -80°C under sterile conditions after reconstitution. Ø 1 month, 2 to 8 °C under sterile conditions after reconstitution. Avoid repeated freeze-thaw cycles. |