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◤反应原理(Test Principle)：
本试产品采用双抗体夹心ELISA法。用抗Human EGFR抗体包被于酶标板上，实验时样品（或标准品）中的Human EGFR会与包被抗体结合。后依次加入生物素化的抗Human EGFR抗体和HRP酶标记的亲和素，抗Human EGFR抗体与结合在包被抗体上的Human EGFR结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在HRP酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，Human EGFR浓度与OD450 OD值之间呈正比，通过绘制标准曲线计算出样品中Human EGFR的浓度。
◤存储(Storage)：
Store the product at 2-8°C before unopened. Upon receipt, unpack promptly and store as recommended in the instructions. | ◤关键信息(Key Features):
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◤实验过程(Assay Procedures):
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| ◤典型数据(Typical Data): | |
 | Human EGFR ELISA Standard Curve Typical data are for reference only and curves should be replotted for each experiment. The Logistics function is recommended for fitting. |
◤精密度(Precision):
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Intra-assay precision (intra-assay precision): Low, medium, and high levels of Human EGFR were measured on a single plate 20 times at each level. Inter-assay Precision (interassay precision): Samples containing low, intermediate, and high levels of Human EGFR were assayed 20 times per plate on 3 different plates. | ||||||||||||||||||||||||||||||||||||||||||
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◤回收率(Recovery):
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| Three different levels of Human EGFR spiked in samples were evaluated for recovery in different matrices. | ||||||||||||
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◤线性(Linearity):
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| A high concentration of Human EGFR is added to the sample, which is then diluted with a standard and a sample diluent to bring the value of the sample within the detection range. |
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◤组份清单(Component List):
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