The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
基因名称
GAPDH
免疫原
Synthetic Peptide of GAPDH
特异性
The antibody detects endogenous GAPDH protein.
稀释度
WB 1:5000-20000, IHC-P 1:200-300, IF-ICC 1:200
宿主
Monoclonal, Mouse
存储缓冲液
PBS, pH 7.4, containing 0.5%BSA, 0.02% New type preservative N as Preservative and 50% Glycerol.
运输及保存
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
背景介绍
Glyceraldehyde-3-phosphate dehydrogenase(GAPDH) Homo sapiens This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, etc.