| 产品名称 | GMP Recombinant Human Fibronectin |
| 蛋白名 | Fibronectin |
| 纯度 | Greater than 95% as determined by reducing SDS-PAGE |
| 内毒素 | ≤10 EU/mg |
| 蛋白构建 | Recombinant Human Fibronectin is produced by our Mammalian cell expression system and the target gene encoding Pro1270-Ser1546&Ala1721-Thr2016 is expressed. |
| Accession # | P02751 |
| 蛋白标签 | Tag free |
| 表达宿主 | Mammalian cell |
| 种属 | Human |
| 预测分子量 | 62.6 kDa |
| 蛋白形态 | Lyophilized |
| 储存缓冲液 | PBS,5% mannitol and 0.01% Tween 80, pH7.4 |
| 运输方式 | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below. |
| 稳定性&储存 | Store at ≤-70°C, stable for 6 months after receipt. Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles. |
| 复溶 | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
分子别名
FN; Cold-insoluble globulin; CIG; FN; Fibronectin 1
蛋白活性
The ED50 for this effect is 35.0-420 ng/mL
背景介绍
Fibronectin1(FN1) is a secreted protein and contains 12 fibronectin type-I domains,fibronectin type-II domains and 16 fibronectin type-III domains.Recombinant human fibronectin fragment, is a protein of ~63 kDa containing a central cell-binding domain, a high affinity heparin-binding domain II,and CS1 site within the alternatively spliced III CS region of human fibronectin. Cells bind to a VLA-4 ligand, a CS-I site, and a VLA-5 ligand, a cell attachment domain, and virus vectors binds to a heparin binding domain II, which co-locates the cell and the virus vector on NovoNectin. This process enhances the density of both cells and vectors, and facilitates the gene transduction in the result.
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