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Trypanothione synthetase-IN-4 is a L. infantumTryS inhibitor whose activity is dependent on the concentration of the polyamine substrate. trypanothione synthetase-IN-4 exhibits a strong anti-leishmanicidal effect (EC50: 0.6 μM, SI ( Trypanothione synthetase-IN-4 can be used to study leishmaniasis.