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DHFR-IN-1 is a selective and potent inhibitor of DHFR (dihydrofolate reductase) (IC50: 40.71 nM). DHFR-IN-1 exhibited some antifungal activity and good antibacterial activity against Gram-positive and Gram-negative bacteria.DHFR-IN-1 showed good synergy with Levofloxacin with FIC (partial inhibitory concentration index) = 0.249.