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JAK3/BTK-IN-3 is a potent inhibitor of JAK3/BTK. BTK and JAK3 are two important targets in autoimmune diseases. Simultaneous inhibition of the BTK/JAK3 signalling pathway has a synergistic effect and JAK3/BTK-IN-3 has shown potential for the investigation of JAK3 kinases and/or BTK-related diseases.