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HF50731 is a potent antagonist of CXCR4. HF50731 has a strong binding affinity for CXCR4 (IC50: 19.8 nM). HF50731 utilizes the CXCR4 co-receptor and effectively inhibits calcium mobilization (IC50: 119.2 nM), cell migration (IC50: 621.4 nM) and HIV-1 infection (IC50: 1.5 nM). IC50: 1.5 nM).