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SK-575-NEG (compound 28), a methylated derivative of SK-575, results from the methylation of the piperidine-2,6-dione's amino group in SK-575, serving as a control compound. It exhibits strong binding affinity to PARP1, evidenced by an IC50 of 2.64 nM. However, SK-575-NEG proved to be ineffective in promoting PARP1 degradation in MDA-MB-436 and Capan-1 cells, even at concentrations as high as 1 μM [1].