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Metallo-β-lactamase-IN-3 (compound 35) is a potent metallo-β-lactamases (MBL) inhibitor that has the potential to restore the activity of current β-lactam antibiotics and to offer an orthogonal strategy to the discovery of new antibiotics. Metallo-β-lactamase-IN-3 shows high activity against VIM-1 and NDM-1, with IC 50 of 0.6 and 1.0 μM, respectively. Metallo-β-lactamase-IN-3 does not show inhibition of IMP-7 [1].