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9-cis-Retinal is a retinoid with Kd of 8 nM and 5 nM for cellular retinol binding protein-I (CRBP-1) and CRBP-II, respectively. 9-cis-Retinal accelerates the differentiation and maturation of rod photoreceptors in retinal organoids, reduces the toxicity of unliganded opsins, and can be used to study congenital stationary night blindness caused by G90D rhodopsin.