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Rabbit polyclonal antibody to MKK4
产品名:MAP2K4 antibody
货号:orb318935
产品中文名:MAP2K4 antibody 抗体
产品详情链接:
https://www.biorbyt.com/map2k4-antibody-orb318935.htmlImmunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human MKK4. The exact sequence is proprietary.
Tested Applications: IF, IH, WB
Application Dilutions: WB: 1:500-1:1000
Reacts With: Human, Mouse, Primate, Rat, Zebrafish
Conjugation: Unconjugated
Host: Rabbit
Clonality: Polyclonal
Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Preservative: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Source: Rabbit
Storage Instructions: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
Alternative Names: anti JNKK1 antibody, anti MEK4 antibody, anti MKK4 antibody, anti PRKMK4 antibody, anti SEK1 antibody, anti SERK1 antibody, anti SKK1 antibody, anti Dual specificity mitogen-activated protein kinase kinase 4 antibody, anti MAP kinase kinase 4 antibody, anti MAPKK 4 antibody, anti JNK-activating kinase 1 antibody, anti MAPK/ERK kinase 4 antibody, anti MEK 4 antibody, anti SAPK/ERK kinase 1 antibody, anti SEK1 antibody, anti Stress-activated protein kinase kinase 1 antibody, anti SAPK kinase 1 antibody, anti SAPKK-1 antibody, anti SAPKK1 antibody, anti c-Jun N-terminal kinase kinase 1 antibody, anti JNKK antibody
Entrez: 26398, 6416
UniProt ID: P47809, P45985
Target: MAP2K4
Note: For research use only.
Immunofluorescent analysis of MKK4 staining in COS7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Immunohistochemical analysis of MKK4 staining in human skeletal muscle formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blot analysis of MKK4 expression in Hela (A), A2780 (B), mouse muscle (C), rat muscle (D) whole cell lysates. (Predicted band size: 44 kD; Observed band size: 44 kD)
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