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Rabbit polyclonal antibody to JNK1/2/3 (Phospho-T183/Y185)
产品名:JNK1/2/3 (Phospho-T183/Y185) antibody
货号:orb315601
产品中文名:JNK1/2/3 (Phospho-T183/Y185) antibody 抗体
产品详情链接:
https://www.biorbyt.com/jnk123-phospho-t183y185-a-orb315601.htmlImmunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding T183/Y185 of human JNK1/2/3 protein. The exact sequence is proprietary.
Tested Applications: IF, WB
Application Dilutions: WB: 1-500:1000
Reacts With: Human, Mouse, Rat
Conjugation: Unconjugated
Host: Rabbit
Clonality: Polyclonal
Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Preservative: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Source: Rabbit
Storage Instructions: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
Alternative Names: anti MAPK8 antibody, anti JNK1 antibody, anti PRKM8 antibody, anti SAPK1 antibody, anti SAPK1C antibody, anti Mitogen-activated protein kinase 8 antibody, anti MAP kinase 8 antibody, anti MAPK 8 antibody, anti JNK-46 antibody, anti Stress-activated protein kinase 1c antibody, anti SAPK1c antibody, anti Stress-activated protein kinase JNK1 antibody, anti c-Jun N-terminal kinase 1 antibody, anti MAPK9 antibody, anti JNK2 antibody, anti PRKM9 antibody, anti SAPK1A antibody, anti Mitogen-activated protein kinase 9 antibody, anti MAP kinase 9 antibody, anti MAPK 9 antibody, anti JNK-55 antibody, anti Stress-activated protein kinase 1a antibody, anti SAPK1a antibody, anti Stress-activated protein kinase JNK2 antibody, anti c-Jun N-terminal kinase 2 antibody, anti MAPK10 antibody, anti JNK3 antibody, anti JNK3A antibody, anti PRKM10 antibody, anti SAPK1B antibody, anti Mitogen-activated protein kinase 10 antibody, anti MAP kinase 10 antibody, anti MAPK 10 antibody, anti MAP kinase p49 3F12 antibody, anti Stress-activated protein kinase 1b antibody, anti SAPK1b antibody, anti Stress-activated protein kinase JNK3 antibody, anti c-Jun N-terminal kinase 3 antibody
Entrez: 25272, 26419, 116554, 5602, 5599, 5601, 26420, 50658
UniProt ID: P45984, P49185, P53779, Q9WTU6, Q61831, P49186, P49187, Q91Y86, P45983
Target: MAPK8; MAPK9; MAPK10
Note: For research use only.
Immunofluorescent analysis of JNK1/2/3 (Phospho-T183/Y185) staining in LS8 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
Western blot analysis of JNK1/2/3 (Phospho-T183/Y185) expression in BV2 (A), MCF7 (B), HCT116 (C), U87MG (D) whole cell lysates. (Predicted band size: 48; 52 kD; Observed band size: 46; 54 kD)
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