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Mouse monoclonal to Mouse IgM Isotype Control

产品名：Mouse IgM Isotype Control Antibody

货号：orb1293411

产品中文名：Mouse IgM Isotype Control Antibody 抗体

产品详情链接：https://www.biorbyt.com/mouse-igm-isotype-control-anti-orb1293411.html

Tested Applications: FC, IF

Application Notes: 1. Take 100 μl peripheral blood anticoagulated by EDTA and add to the bottom of 5 ml tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood; incubate for 30 minutes at room temperature. 3. Add 2 ml RBC lysis buffer; incubate for 10 minutes after mixing; dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes; discard the supernatant. 5. Add 2 ml PBS wash buffer to resuspend the cells; then 1000 rpm centrifugation for 5 minutes; discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 ml PBS wash buffer to resuspend the cells; then 1000 rpm centrifugation for 5 minutes; discard the supernatant. 8. Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Conjugation: Unconjugated

Host: Mouse

Clonality: Monoclonal

Clone: 7C2C6C2

Form/Appearance: Mouse IgM. Liquid in PBS, pH 7.3, and 0.02% sodium azide.

Preservative: Mouse IgM. Liquid in PBS, pH 7.3, and 0.02% sodium azide.

Source: Mouse

Storage Instructions: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Note: For research use only.

英国biorbyt（www.biorbyt.com），成立于2011年，总部位于世界科技中心之一的英国剑桥郡，专注于生命科学和生物技术领域，系英国高品质生命科学试剂供应商。产品涉及各种科研领域，被许多知名杂志引用，并收到客户的良好反馈。