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Rabbit polyclonal antibody to CA9
产品名:CA9 antibody
货号:orb213630
产品中文名:CA9 antibody 抗体
产品详情链接:
https://www.biorbyt.com/ca9-antibody-orb213630.htmlImmunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Carbonic Anhydrase 9. The exact sequence is proprietary.
Tested Applications: IF, WB
Application Dilutions: WB: 1-500-1-1000
Reacts With: Human, Mouse, Rat
Conjugation: Unconjugated
Host: Rabbit
Clonality: Polyclonal
Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Preservative: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Source: Rabbit
Storage Instructions: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
Alternative Names: anti G250 antibody, anti MN antibody, anti Carbonic anhydrase 9 antibody, anti Carbonate dehydratase IX antibody, anti Carbonic anhydrase IX antibody, anti CA-IX antibody, anti CAIX antibody, anti Membrane antigen MN antibody, anti P54/58N antibody, anti Renal cell carcinoma-associated antigen G250 antibody, anti RCC-associated antigen G250 antibody, anti pMW1 antibody
Entrez: 768, 230099
UniProt ID: Q16790, Q8VHB5
Target: CA9
Note: For research use only.
Immunofluorescent analysis of Carbonic Anhydrase 9 staining in HEK293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
Western blot analysis of Carbonic Anhydrase 9 expression in U2OS (A), mouse kidney (B), mouse brain (C), rat brain (D) whole cell lysates. (Predicted band size: 49 kD; Observed band size: 49 kD)
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