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T lymphocyte activating protein antibody
产品名:Rabbit Anti-Ly6a antibody
货号:orb6918
产品中文名:Rabbit Anti-Ly6a antibody 抗体
产品详情链接:
https://www.biorbyt.com/rabbit-anti-ly6a-antibody-orb6918.htmlImmunogen: KLH conjugated synthetic peptide derived from mouse Sca1/Ly6A/E (15-110/134 aa)
Tested Applications: FC
Application Dilutions: Flow-Cyt=2μg /Test, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
Reacts With: Mouse, Rat
Conjugation: Unconjugated
Host: Rabbit
Clonality: Polyclonal
Isotype: IgG
Purity: Affinity purified by Protein A
Molecular Weight: 9 kDa
Form/Appearance: Liquid
Preservative: 0.01M TBS(pH7.4) with 1% rAlbumin, 0.03% Proclin300 and 50% Glycerol
Concentration: 1 mg/ml
Storage Instructions: Shipped at 4°C. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Alternative Names: Lymphocyte antigen 6A-2/6E-1; Ly-6A.2/Ly-6E.1; Stem cell antigen 1; Sca1 / Ly6A/E; SCA-1; T-cell-activating protein; TAP; Ly6al; Sca1; LY6A_MOUSE.
UniProt ID: P05533
Target: Ly6a
Note: For research use only.
Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15min Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer at 37℃ for 20 min; Incubation: Anti-Sca1 Polyclonal Antibody 1:500 overnight at 4°C followed by conjugation to the secondary antibody.
Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH 6.0), Blocking buffer (normal goat serum at 37℃ for 20 min).
Tissue/cell: mouse thymus tissue;4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation: Anti-Sca1 Polyclonal Antibody, Unconjugated 1:500, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated used at 1:200 dilution for 40 minutes at 37°C.
FC Application: Blank control: Mouse Thymus Cells(blue), Primary Antibody: Rabbit Anti-Sca1/FITC, Conjugated antibody, Dilution: 2μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/FITC(orange), used under the same conditions.
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