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TM7SF2 antibody
产品名:TM7SF2 antibody
货号:orb4574
产品中文名:TM7SF2 antibody 抗体
产品详情链接:
https://www.biorbyt.com/tm7sf2-antibody-orb4574.htmlImmunogen: KLH conjugated synthetic peptide derived from human TM7SF2/DHCR14A (51-150/418 aa)
Tested Applications: IHC-P, WB
Application Dilutions: WB=1:500-2000, ELISA=1:5000-10000
Reacts With: Human, Mouse, Rat
Predicted Reactivity: Bovine, Rabbit, Sheep
Conjugation: Unconjugated
Host: Rabbit
Clonality: Polyclonal
Isotype: IgG
Molecular Weight: 46 kDa
Form/Appearance: Liquid
Preservative: 0.01M TBS(pH7.4) with 1% rAlbumin, 0.03% Proclin300 and 50% Glycerol.
Concentration: 1mg/ml
Storage Instructions: Shipped at 4°C. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Alternative Names: ANG1; Another new gene 1 protein; DHCR14A; C 14 sterol reductase; C-14 sterol reductase; Delta 14 SR; Delta(14) sterol reductase; Delta(14)-sterol reductase; Delta-14-SR; DHCR14A; ERG24_HUMAN; NET47; Putative sterol reductase SR 1; Putative sterol reductase SR-1; Sterol C14 reductase; Sterol C14-reductase; TM7SF2; Transmembrane 7 superfamily member 2.
Pubmed: 34667152
UniProt ID: O76062
Target: TM7SF2
References: Xu, Yichi et al. TM7SF2 regulates cell proliferation and apoptosis by activation of C-Raf/ERK pathway in cervical cancer Cell Death Discov, 7, 299 (2021)
Note: For research use only.
Paraformaldehyde-fixed, paraffin embedded (Human prostatic cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (TM7SF2 ) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C
Paraformaldehyde-fixed, paraffin embedded (Human adrenal gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (TM7SF2 ) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C
Paraformaldehyde-fixed, paraffin embedded (Human heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (TM7SF2 ) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C
Sample: Lane 1: Mouse Adrenal gland tissue lysates. Lane 2: Rat Adrenal gland tissue lysates. Primary: Anti-TM7SF2 at 1/1000 dilution. Secondary: Goat Anti-Rabbit IgG at 1/20000 dilution. Predicted band size: 46 kDa. Observed band size: 58 kDa
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