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This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human E2 antigen, and the Human E2 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human E2 to each microplate well and incubated . After TMB substrate solution is added, the enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±10nm. The concentration of Human E2 in the samples is then determined by comparing the OD of the samples to the standard curve.

产品名：EasyStep Human E2(Estradiol) ELISA Kit

货号：orb1950181

产品中文名：EasyStep Human E2(Estradiol) ELISA Kit 酶联免疫试剂盒

产品详情链接：https://www.biorbyt.com/easystep-human-e2estradiol-e-orb1950181.html

Alternative Names: 17B-Estradiol; Oestradiol; Beta-Estradiol

Application Notes: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human E2 antigen, and the Human E2 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human E2 to each microplate well and incubated . After TMB substrate solution is added, the enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±10nm. The concentration of Human E2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Reacts With: Human

Dynamic Range: 25-1600 pg/mL

Sensitivity: 25 pg/mL

Sample Types: Serum

Note: For research use only.

英国biorbyt（www.biorbyt.com），成立于2011年，总部位于世界科技中心之一的英国剑桥郡，专注于生命科学和生物技术领域，系英国高品质生命科学试剂供应商。产品涉及各种科研领域，被许多知名杂志引用，并收到客户的良好反馈。