已关闭
收藏ICC shows positive staining in HeLa cells. Anti-Vimentin (Alexa Fluor® 647 Conjugate) antibody was used at 1/250 dilution (magenta) and incubated overnight at 4°C. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nucl