描述：

Image-iT Hypoxia Reagent是一种新型的、可固定的荧光标记物，专用于测定活细胞中的缺氧状态。当活细胞处于正常氧浓度的环境中时，它是不发荧光的; 当氧浓度降低时，它的荧光亮度会变亮，可用于实时动态监测低氧下的细胞状态。
可提供两款缺氧检测试剂 Image-iT™ Green Hypoxia Reagent (I14833, I14834) 和 Image-iT™ Red Hypoxia Reagent (H10498):

• 可动态监测：低氧环境下的活细胞；
• 两种颜色可选： Image-iT™ Red Hypoxia 和 Image-iT™ Green Hypoxia；
• 可固定：Image-iT Green Hypoxia 可固定但不可逆,而Image-iT™ Red Hypoxia可进行缺氧可逆检测；
• 简便易用：直接加入培养细胞中即可成像；

Analyzing HeLa cells under hypoxic condition CellLight Histone 2B-RFP
HeLa cells were plated on MatTek dishes at a density of 100k/dish and incubated with CellLight Histone 2B-RFP (red) overnight at 37°C in CO₂ incubator. Medium was replaced with fresh growth medium containing Image-iT Green Hypoxia Reagent at a final concentration of 5 µM. Cells were incubated at 20% or 1% oxygen for 3 hrs. The cells were then washed 2X with Live Cell Imaging Solution (LCIS) and incubated with and NucBlue Live nuclear stain (blue). Cells were imaged on an EVOS FL Auto Cell Imaging System.



Image-iT Green Hypoxia Reagent is a novel, fixable fluorogenic compound for measuring hypoxia in live cells. It is non-fluorescent when live cells are in an environment with normal oxygen concentrations and becomes fluorescent when oxygen levels are decreased. Image-iT Green Hypoxia Reagent sustains its fluorescence when cells/tissue return to normal oxygen levels, allowing the cells/tissue to be fixed with minimal loss of fluorescent signal.

Features of Image-iT Green Hypoxia Reagent include:
• Indicates hypoxia in live cells by fluorescing in low oxygen environments
• Fixable—sustains fluorescent signal when cells/tissue return to normal oxygen levels
• Easy to use—just add to cell culture media and image

Image-iT Green Hypoxia Reagent is a fluorogenic compound that is live cell permeable and becomes fluorescent in environments with low oxygen concentrations. These properties makes it a highly useful tool for detecting cells and tissue under hypoxic conditions. Image-iT Green Hypoxia Reagent is a very sensitive oxygen detector. Unlike pimonidazole adducts that respond only to very low oxygen levels, Image-iT Green Hypoxia Reagent begins to fluoresce when atmospheric oxygen levels are less than 5%. It responds quickly to such environments, and the fluorescence is sustained after the oxygen levels return to normal. These properties make Image-iT Green Hypoxia Reagent an ideal tool for detecting hypoxic conditions around tumors, 3D cultures, spheroids, neurons, etc.

For Research Use Only. Not for use in diagnostic procedures.