Interleukin-8 (IL-8), a member of a family of structurally related cytokines, is a neutrophil chemotactic cytokine that can signal through the CXCR1 and CXCR2 receptor. It is proinflammatory and primarily mediates the activation and migration of neutrophils from peripheral blood into the sites of inflammation, injury, or infection in the tissue. IL-8 is involved in a wide variety of physiological and pathological processes, including host defense against bacterial infection, bronchiolitis, arteriosclerosis, autoimmune disorders of skin, and angiogenesis-dependent disorders such as tumor growth, and wound repair. IL-8 is produced by stimulated monocytes, leukocytic cells, non-leukocytic somatic cells and tumor cells.
GenScript Human IL-8 ELISA Detection Kit is a solid phase sandwich ELISA for quantitative measurement of human IL-8 in serum, plasma and cell culture supernates. It utilizes a mouse anti-human IL-8 monoclonal antibody coated on 96-well plate as capture antibody. Standards and samples are pipetted into the wells and the IL-8 present is bound by the immobilized antibody. After washing away any unbound substances, another mouse anti-Human IL-8 monoclonal Antibody with HRP conjugation as detection antibody to specific bind human IL-8. After washing again, TMB substrate solution is added and the color developed is proportional to the amount of IL-8 present in the testing samples.