Mycoplasma contamination is common in most cell culture and tissue culture laboratories. In biological drug safety control, most of pharmacopeias, including EP, USP, and JP, recommend using nucleic acid amplification testing (NAT) as a complementary or alternative method for mycoplasma release test. ResiQuant® Mycoplasma Detection Kit (Taqman) targeting the highly conserved gene fragment of mycoplasma, is able to detect approximately 202 species including the eight specified in EP 2.6.7 for the assay validation. The performance has been validated in accordance with the mycoplasma testing requirements outlined in EP 2.6.7, JP XVIII, and USP <77>. With a limit of detection (LOD) as low as 10 CFU/mL, the kit fulfills the criteria for alternative rapid detection compared with standard 28-day culture method. ● Free of PCR aerosol contamination: Uracil-N-glycosylase (UNG) system is used to reduce the risk of false positive result. ● Quality assurance: A dual-channel detection method is applied, involving PC (positive control) and RC (recovery control), to reduce the risk of false negative result. ● High specificity: No cross-activity to cross-related bacterial species and industrial mammalian cells.