产品描述
This Human β-TG ELISA Kit was designed for the quantitative measurement of Human β-TG protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.78 ng/mL-50 ng/mL and the sensitivity is 0.195 ng/mL .
别名
beta-Thromboglobulin, beta-TG
缩写
β-TG
灵敏度
0.195 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Others
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human β-TG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 92 | |
| Range % | 88-97 | |
| 1:2 | Average % | 90 | |
| Range % | 85-95 | |
| 1:4 | Average % | 103 | |
| Range % | 97-109 | |
| 1:8 | Average % | 84 | |
| Range % | 80-89 | |
回收率
| The recovery of human β-TG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 97 | 92-101 | |
| EDTA plasma (n=4) | 95 | 90-99 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| ng/ml | OD1 | OD2 | Average | Corrected | | | 50 | 2.742 | 2.821 | 2.782 | 2.663 | | | 25 | 1.906 | 2.015 | 1.961 | 1.842 | | | 12.5 | 1.203 | 1.274 | 1.239 | 1.120 | | | 6.25 | 0.657 | 0.703 | 0.680 | 0.561 | | | 3.12 | 0.435 | 0.404 | 0.420 | 0.301 | | | 1.56 | 0.325 | 0.319 | 0.322 | 0.203 | | | 0.78 | 0.205 | 0.191 | 0.198 | 0.079 | | | 0 | 0.122 | 0.115 | 0.119 | | |
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货期
3-5 working days