产品描述
This Monkey VEGF ELISA Kit was designed for the quantitative measurement of Monkey VEGF protein in serum, plasma, cell culture supernates, cell lysates. It is a Sandwich ELISA kit, its detection range is 125 pg/mL-8000 pg/mL and the sensitivity is 31.25 pg/mL.
缩写
VEGF
灵敏度
31.25 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
| | | | | | | |
标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 8000 | 2.422 | 2.276 | 2.349 | 2.206 | | | 4000 | 2.048 | 1.954 | 2.001 | 1.858 | | | 2000 | 1.443 | 1.511 | 1.477 | 1.334 | | | 1000 | 0.746 | 0.777 | 0.762 | 0.619 | | | 500 | 0.472 | 0.467 | 0.470 | 0.327 | | | 250 | 0.358 | 0.366 | 0.362 | 0.219 | | | 125 | 0.241 | 0.234 | 0.238 | 0.095 | | | 0 | 0.145 | 0.141 | 0.143 | | |
|
货期
3-5 working days