产品描述

This Human YWHAZ ELISA Kit was designed for the quantitative measurement of Human YWHAZ protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.625 ng/mL-40 ng/mL and the sensitivity is 0.156 ng/mL.

别名

14 3 3 delta ELISA Kit; 14 3 3 protein zeta/delta ELISA Kit; 14 3 3 protein/cytosolic phospholipase A2 ELISA Kit; 14 3 3 zeta ELISA Kit; 14-3-3 protein zeta/delta ELISA Kit; 1433Z_HUMAN ELISA Kit; Epididymis luminal protein 4 ELISA Kit; Epididymis secretory protein Li 3 ELISA Kit; HEL S 3 ELISA Kit; HEL4 ELISA Kit; KCIP-1 ELISA Kit; KCIP1 ELISA Kit; MGC111427 ELISA Kit; MGC126532 ELISA Kit; MGC138156 ELISA Kit; Phospholipase A2 ELISA Kit; Protein kinase C inhibitor protein 1 ELISA Kit; Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein; delta polypeptide ELISA Kit; Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein; zeta ELISA Kit; Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein; zeta polypeptide ELISA Kit; Tyrosine 3/tryptophan 5 monooxygenase activation protein; zeta polypeptide ELISA Kit; YWHAD ELISA Kit; YWHAZ ELISA Kit

缩写

YWHAZ

Uniprot No

P63104

灵敏度

0.156 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Others

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human YWHAZ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		93
		Range %		89-99
1:2		Average %		89
		Range %		85-95
1:4		Average %		97
		Range %		90-105
1:8		Average %		95
		Range %		87-100

回收率

The recovery of human YWHAZ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		97		92-103
EDTA plasma (n=4)		92		88-95

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected   40 2.454 2.488 2.471 2.334   20 2.049 2.019 2.034 1.897   10 1.441 1.394 1.418 1.281   5 0.908 0.868 0.888 0.751   2.5 0.595 0.557 0.576 0.439   1.25 0.352 0.356 0.354 0.217   0.625 0.265 0.255 0.260 0.123   0 0.136 0.137 0.137

货期

3-5 working days