产品描述
This Mouse β-EP ELISA Kit was designed for the quantitative measurement of Mouse β-EP protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.78 pg/mL-50 pg/mL and the sensitivity is 0.195 pg/mL .
别名
beta-Endorphin, beta-EP
缩写
β-EP
灵敏度
0.195 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cancer
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse β-EP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 89 | |
| Range % | 86-92 | |
| 1:2 | Average % | 95 | |
| Range % | 91-98 | |
| 1:4 | Average % | 101 | |
| Range % | 97-104 | |
| 1:8 | Average % | 92 | |
| Range % | 89-95 | |
回收率
| The recovery of mouse β-EP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 95 | 91-98 | |
| EDTA plasma (n=4) | 94 | 90-97 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 50 | 2.284 | 2.298 | 2.291 | 2.099 | | | 25 | 1.782 | 1.788 | 1.785 | 1.593 | | | 12.5 | 1.141 | 1.169 | 1.155 | 0.963 | | | 6.25 | 0.638 | 0.601 | 0.620 | 0.428 | | | 3.12 | 0.459 | 0.436 | 0.448 | 0.256 | | | 1.56 | 0.369 | 0.342 | 0.356 | 0.164 | | | 0.78 | 0.298 | 0.284 | 0.291 | 0.099 | | | 0 | 0.199 | 0.185 | 0.192 | | |
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货期
3-5 working days