产品描述

This Human α-GST ELISA Kit was designed for the quantitative measurement of Human α-GST protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.

别名

Glutathione S alkyltransferase A1 ELISA Kit; Glutathione S aryltransferase A1 ELISA Kit; Glutathione S transferase 2 ELISA Kit; Glutathione S transferase A1 ELISA Kit; Glutathione S transferase alpha 1 ELISA Kit; Glutathione S transferase Ha subunit 1 ELISA Kit; Glutathione S-transferase A1 ELISA Kit; GST class-alpha member 1 ELISA Kit; GST epsilon ELISA Kit; GST HA subunit 1 ELISA Kit; GST; class alpha; 1 ELISA Kit; GST-epsilon ELISA Kit; GST2 ELISA Kit; GSTA1 1 ELISA Kit; GSTA1 ELISA Kit; GSTA1-1 ELISA Kit; GSTA1_HUMAN ELISA Kit; GTH1 ELISA Kit; HA subunit 1 ELISA Kit; MGC131939 ELISA Kit; OTTHUMP00000016611 ELISA Kit; S (hydroxyalkyl)glutathione lyase A1 ELISA Kit

缩写

α-GST

Uniprot No

P08263

灵敏度

0.039 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Metabolism

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human α-GST in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		102
		Range %		98-109
1:2		Average %		86
		Range %		81-90
1:4		Average %		93
		Range %		89-98
1:8		Average %		90
		Range %		85-94

回收率

The recovery of human α-GST spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		95		90-100
EDTA plasma (n=4)		88		84-92

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected 10 2.716 2.629 2.673 2.559 5 2.071 2.002 2.037 1.923 2.5 1.482 1.567 1.525 1.411 1.25 0.904 0.961 0.933 0.819 0.625 0.589 0.623 0.606 0.492 0.312 0.365 0.384 0.375 0.261 0.156 0.259 0.263 0.261 0.147 0 0.113 0.115 0.114

货期

3-5 working days