产品描述
This Bovine β-EP ELISA Kit was designed for the quantitative measurement of Bovine β-EP protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 46.88 pg/mL-3000 pg/mL and the sensitivity is 11.72 pg/mL .
别名
beta-Endorphin, beta-EP
缩写
β-EP
灵敏度
11.72 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cancer
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of bovine β-EP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 95 | |
| Range % | 90-99 | |
| 1:2 | Average % | 84 | |
| Range % | 80-88 | |
| 1:4 | Average % | 92 | |
| Range % | 88-97 | |
| 1:8 | Average % | 90 | |
| Range % | 85-94 | |
回收率
| The recovery of bovine β-EP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 99 | 95-104 | |
| EDTA plasma (n=4) | 92 | 88-96 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 3000 | 1.701 | 1.612 | 1.657 | 1.559 | | | 1500 | 1.376 | 1.458 | 1.417 | 1.319 | | | 750 | 1.113 | 1.104 | 1.109 | 1.011 | | | 375 | 0.832 | 0.809 | 0.821 | 0.723 | | | 187.5 | 0.524 | 0.560 | 0.542 | 0.444 | | | 93.75 | 0.367 | 0.359 | 0.363 | 0.265 | | | 46.88 | 0.264 | 0.251 | 0.258 | 0.160 | | | 0 | 0.099 | 0.097 | 0.098 | | |
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货期
3-5 working days