产品描述
This Rat β-EP ELISA Kit was designed for the quantitative measurement of Rat β-EP protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 10 pg/mL-2000 pg/mL and the sensitivity is 5 pg/mL.
别名
beta-Endorphin, beta-EP
缩写
β-EP
灵敏度
5 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cancer
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<15% |
|
|
|
| Three samples of known concentration were tested twenty times on one plate to assess. |
|
| Inter-assay Precision (Precision between assays): CV%<15% |
|
|
|
| Three samples of known concentration were tested in twenty assays to assess. |
|
|
| |
|
|
|
|
|
|
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat β-EP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| |
Sample |
Serum(n=4) |
|
| 1:1 |
Average % |
84 |
|
| Range % |
80-90 |
|
| 1:2 |
Average % |
97 |
|
| Range % |
91-105 |
|
| 1:4 |
Average % |
94 |
|
| Range % |
90-98 |
|
| 1:8 |
Average % |
92 |
|
| Range % |
88-96 |
|
回收率
| The recovery of rat β-EP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
95 |
89-98 |
|
| EDTA plasma (n=4) |
90 |
84-96 |
|
| |
|
|
|
|
|
|
| |
|
|
|
|
|
|
标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
|
|
| 2000 |
0.214 |
0.212 |
0.213 |
|
|
| 500 |
0.365 |
0.336 |
0.351 |
|
|
| 160 |
0.534 |
0.578 |
0.556 |
|
|
| 40 |
0.939 |
0.994 |
0.967 |
|
|
| 10 |
1.424 |
1.476 |
1.450 |
|
|
| 0 |
2.381 |
2.259 |
2.320 |
|
|
|
货期
3-5 working days