产品描述
This Mouse VEGFB ELISA Kit was designed for the quantitative measurement of Mouse VEGFB protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 7.8 pg/mL-500 pg/mL and the sensitivity is 0.195 pg/mL .
别名
Vegfb ELISA kit; VrfVascular endothelial growth factor B ELISA kit; VEGF-B ELISA kit; VEGF-related factor ELISA kit; VRF ELISA kit
缩写
VEGFB
Uniprot No
P49766
灵敏度
0.195 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cancer
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse VEGF-B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 97 | |
| Range % | 92-102 | |
| 1:2 | Average % | 91 | |
| Range % | 84-97 | |
| 1:4 | Average % | 90 | |
| Range % | 87-94 | |
| 1:8 | Average % | 100 | |
| Range % | 95-103 | |
回收率
| The recovery of mouse VEGF-B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 90 | 87-93 | |
| EDTA plasma (n=4) | 93 | 90-96 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 500 | 2.743 | 2.763 | 2.753 | 2.589 | | | 250 | 2.405 | 2.435 | 2.420 | 2.256 | | | 125 | 1.756 | 1.796 | 1.776 | 1.612 | | | 62.5 | 0.978 | 1.016 | 0.997 | 0.833 | | | 31.2 | 0.501 | 0.535 | 0.518 | 0.354 | | | 15.6 | 0.351 | 0.359 | 0.355 | 0.191 | | | 0.78 | 0.237 | 0.255 | 0.246 | 0.082 | | | 0 | 0.162 | 0.165 | 0.164 | | |
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货期
3-5 working days