产品描述
This Human VEGFB ELISA Kit was designed for the quantitative measurement of Human VEGFB protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.
别名
Vascular endothelial growth factor B ELISA Kit; Vascular endothelial growth factor-related factor ELISA Kit; VEGF B ELISA Kit; VEGF related factor ELISA Kit; VEGF-B ELISA Kit; VEGF-related factor ELISA Kit; Vegfb ELISA Kit; VEGFB_HUMAN ELISA Kit; VEGFL ELISA Kit; VRF ELISA Kit
缩写
VEGFB
Uniprot No
P49765
灵敏度
3.9 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cancer
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human VEGF-B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 88 | |
| Range % | 80-96 | |
| 1:2 | Average % | 100 | |
| Range % | 95-104 | |
| 1:4 | Average % | 99 | |
| Range % | 92-110 | |
| 1:8 | Average % | 89 | |
| Range % | 86-96 | |
回收率
| The recovery of human VEGF-B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 99 | 95-105 | |
| EDTA plasma (n=4) | 96 | 92-100 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| pg/ml | OD1 | OD2 | Average | Corrected | | | 1000 | 2.697 | 2.512 | 2.605 | 2.463 | | | 500 | 2.322 | 2.201 | 2.262 | 2.120 | | | 250 | 1.546 | 1.498 | 1.522 | 1.380 | | | 125 | 0.815 | 0.789 | 0.802 | 0.660 | | | 62.5 | 0.417 | 0.410 | 0.414 | 0.272 | | | 31.2 | 0.281 | 0.276 | 0.279 | 0.137 | | | 15.6 | 0.219 | 0.213 | 0.216 | 0.074 | | | 0 | 0.143 | 0.140 | 0.142 | | |
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货期
3-5 working days