产品描述

This Human YWHAH ELISA Kit was designed for the quantitative measurement of Human YWHAH protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.625 ng/mL-40 ng/mL and the sensitivity is 0.156 ng/mL.

别名

14 3 3 protein eta ELISA Kit; 14-3-3 eta ELISA Kit; 14-3-3 protein eta ELISA Kit; 1433F_HUMAN ELISA Kit; Brain protein 14-3-3; eta isoform ELISA Kit; HGNC:12853 ELISA Kit; Protein AS1 ELISA Kit; Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein 1 ELISA Kit; Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein eta polypeptide ELISA Kit; Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein; eta isoform ELISA Kit; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein; eta ELISA Kit; Tyrosine 3/tryptophan 5 monooxygenase activation protein eta polypeptide ELISA Kit; YWHA 1 ELISA Kit; YWHA1 ELISA Kit; Ywhah ELISA Kit

缩写

YWHAH

Uniprot No

Q04917

灵敏度

0.156 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Others

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human YWHAH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		93
		Range %		88-97
1:2		Average %		97
		Range %		93-103
1:4		Average %		85
		Range %		81-89
1:8		Average %		102
		Range %		86-105

回收率

The recovery of human YWHAH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		92		86-98
EDTA plasma (n=4)		88		82-94

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected 40 2.632 2.756 2.694 2.592 20 2.224 2.376 2.300 2.198 10 1.646 1.748 1.697 1.595 5 0.934 0.950 0.942 0.840 2.5 0.524 0.508 0.516 0.414 1.25 0.356 0.362 0.359 0.257 0.625 0.242 0.245 0.244 0.142 0 0.103 0.101 0.102

货期

3-5 working days