产品描述
VEGFA(vascular endothelial growth factor A)是一种促血管生成因子,能促进血管内皮细胞增殖、迁移和管腔形成。它在血管生成和血管再生中扮演重要的角色,并参与多种疾病的发生和发展。VEGFA含量的变化与各种疾病密切相关,如在肿瘤、糖尿病视网膜病变等疾病中表达水平升高。
华美生物所提供的Human Vascular Endothelial cell Growth Factor(VEGF) ELISA KIT属于ELISA检测试剂盒,采用双抗夹心法定量检测人血清、血浆、细胞培养上清、组织匀浆、细胞裂解液、尿液样本中的VEGFA,其灵敏度为25.297 pg/mL,检测范围为31.25 pg/mL-2000 pg/mL。
别名
glioma-derived endothelial cell mitogen ELISA Kit; MGC70609 ELISA Kit; MVCD1 ELISA Kit; Vascular endothelial growth factor A ELISA Kit; Vascular Endothelial Growth Factor ELISA Kit; Vascular permeability factor ELISA Kit; VEGF ELISA Kit; VEGF-A ELISA Kit; VEGF165 ELISA Kit; Vegfa ELISA Kit; VEGFA_HUMAN ELISA Kit; VPF ELISA Kit
缩写
VEGFA
Uniprot No
P15692
灵敏度
25.297 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cancer
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human VEGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
98 |
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| Range % |
94-106 |
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| 1:2 |
Average % |
93 |
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| Range % |
86-97 |
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| 1:4 |
Average % |
87 |
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| Range % |
82-93 |
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| 1:8 |
Average % |
96 |
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| Range % |
91-105 |
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回收率
| The recovery of human VEGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
89 |
85-93 |
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| EDTA plasma (n=4) |
92 |
90-96 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 2000 |
2.651 |
2.751 |
2.701 |
2.547 |
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| 1000 |
1.729 |
1.829 |
1.779 |
1.625 |
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| 500 |
1.067 |
1.087 |
1.077 |
0.923 |
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| 250 |
0.579 |
0.589 |
0.584 |
0.430 |
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| 125 |
0.372 |
0.382 |
0.377 |
0.223 |
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| 62.5 |
0.255 |
0.265 |
0.260 |
0.106 |
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| 31.25 |
0.207 |
0.209 |
0.208 |
0.054 |
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| 0 |
0.153 |
0.154 |
0.154 |
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货期
3-5 working days