产品描述
This Mouse α-MSH ELISA Kit was designed for the quantitative measurement of Mouse α-MSH protein in serum, plasma, cell culture supernates. It is a Sandwich ELISA kit, its detection range is 12.5 pg/mL-800 pg/mL and the sensitivity is 3.12 pg/mL.
别名
alpha-melanocyte stimulating hormone, alpha-MSH
缩写
α-MSH
灵敏度
3.12 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Biochemicals
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse α-MSH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 89 | |
| Range % | 86-95 | |
| 1:2 | Average % | 92 | |
| Range % | 88-99 | |
| 1:4 | Average % | 94 | |
| Range % | 90-99 | |
| 1:8 | Average % | 93 | |
| Range % | 87-98 | |
回收率
| The recovery of mouse α-MSH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 93 | 89-97 | |
| EDTA plasma (n=4) | 95 | 91-99 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 800 | 2.873 | 2.801 | 2.837 | 2.678 | | | 400 | 2.268 | 2.245 | 2.257 | 2.098 | | | 200 | 1.654 | 1.558 | 1.606 | 1.447 | | | 100 | 0.897 | 0.867 | 0.882 | 0.723 | | | 50 | 0.581 | 0.562 | 0.572 | 0.413 | | | 25 | 0.387 | 0.366 | 0.377 | 0.218 | | | 12.5 | 0.298 | 0.284 | 0.291 | 0.132 | | | 0 | 0.161 | 0.157 | 0.159 | | |
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货期
3-5 working days