产品描述
VEGFA(血管内皮生长因子A,vascular endothelial growth factor A)是血管生成和血管修复过程中的重要因子,能促进血管内皮细胞增殖和血管生成。
华美生物所提供的Rat Vascular Endothelial cell Growth Factor,VEGF ELISA Kit属于ELISA检测试剂盒,采用双抗夹心法定量检测大鼠血清、血浆、组织匀浆样本中的VEGFA,其灵敏度为0.97 pg/mL,检测范围为3.9 pg/mL-250 pg/mL。
别名
Vegfa ELISA Kit; Vegf ELISA Kit; Vascular endothelial growth factor A ELISA Kit; VEGF-A ELISA Kit; Vascular permeability factor ELISA Kit; VPF ELISA Kit
缩写
VEGFA
Uniprot No
P16612
灵敏度
0.97 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cancer
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
|
|
|
| Three samples of known concentration were tested twenty times on one plate to assess. |
|
| Inter-assay Precision (Precision between assays): CV%<10% |
|
|
|
| Three samples of known concentration were tested in twenty assays to assess. |
|
|
| |
|
|
|
|
|
|
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat VEGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| |
Sample |
Serum(n=4) |
|
| 1:1 |
Average % |
93 |
|
| Range % |
84-101 |
|
| 1:2 |
Average % |
94 |
|
| Range % |
90-100 |
|
| 1:4 |
Average % |
96 |
|
| Range % |
91-103 |
|
| 1:8 |
Average % |
94 |
|
| Range % |
86-100 |
|
回收率
| The recovery of rat VEGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
94 |
90-97 |
|
| EDTA plasma (n=4) |
97 |
92-102 |
|
| |
|
|
|
|
|
|
| |
|
|
|
|
|
|
标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 250 |
2.712 |
2.892 |
2.802 |
2.614 |
|
| 125 |
2.369 |
2.317 |
2.343 |
2.155 |
|
| 62.5 |
1.568 |
1.604 |
1.586 |
1.398 |
|
| 31.25 |
0.877 |
0.888 |
0.883 |
0.695 |
|
| 15.6 |
0.558 |
0.562 |
0.560 |
0.372 |
|
| 7.8 |
0.371 |
0.384 |
0.378 |
0.190 |
|
| 3.9 |
0.276 |
0.286 |
0.281 |
0.093 |
|
| 0 |
0.188 |
0.187 |
0.188 |
|
|
|
货期
3-5 working days