产品描述
This Human XBP1 ELISA Kit was designed for the quantitative measurement of Human XBP1 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 12.5 pg/mL-800 pg/mL and the sensitivity is 3.12 pg/mL.
别名
Tax responsive element binding protein 5 ELISA Kit; Tax-responsive element-binding protein 5 ELISA Kit; TREB5 ELISA Kit; X box binding protein 1 ELISA Kit; X box binding protein 2 ELISA Kit; X-box-binding protein 1 ELISA Kit; XBP 1 ELISA Kit; XBP-1 ELISA Kit; XBP1 ELISA Kit; XBP1_HUMAN ELISA Kit; XBP2 ELISA Kit
缩写
XBP1
Uniprot No
P17861
灵敏度
3.12 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Epigenetics and Nuclear Signaling
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human XBP1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
96 |
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| Range % |
88-100 |
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| 1:2 |
Average % |
101 |
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| Range % |
93-104 |
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| 1:4 |
Average % |
99 |
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| Range % |
90-104 |
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| 1:8 |
Average % |
95 |
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| Range % |
88-99 |
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回收率
| The recovery of human XBP1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
91 |
83-95 |
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| EDTA plasma (n=4) |
108 |
92-112 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 800 |
2.609 |
2.524 |
2.567 |
2.439 |
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| 400 |
2.069 |
2.011 |
2.040 |
1.912 |
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| 200 |
1.374 |
1.389 |
1.382 |
1.254 |
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| 100 |
0.723 |
0.746 |
0.735 |
0.607 |
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| 50 |
0.470 |
0.461 |
0.466 |
0.338 |
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| 25 |
0.268 |
0.277 |
0.273 |
0.145 |
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| 12.5 |
0.197 |
0.202 |
0.200 |
0.072 |
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| 0 |
0.130 |
0.126 |
0.128 |
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货期
3-5 working days