产品描述

This Human YBX1 ELISA Kit was designed for the quantitative measurement of Human YBX1 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.8 pg/mL.

别名

BP 8 ELISA Kit; CBF-A ELISA Kit; CCAAT binding transcription factor I subunit A ELISA Kit; CCAAT-binding transcription factor I subunit A ELISA Kit; CSDA2 ELISA Kit; CSDB ELISA Kit; DBPB ELISA Kit; DNA binding protein B ELISA Kit; DNA-binding protein B ELISA Kit; EFI-A ELISA Kit; Enhancer factor I subunit A ELISA Kit; MDR NF1 ELISA Kit; MGC104858 ELISA Kit; MGC110976 ELISA Kit; MGC117250 ELISA Kit; NSEP 1 ELISA Kit; NSEP1 ELISA Kit; Nuclease sensitive element binding protein 1 ELISA Kit; Nuclease-sensitive element-binding protein 1 ELISA Kit; p50 ELISA Kit; Q15905 ELISA Kit; Y-box binding protein 1 ELISA Kit; Y-box transcription factor ELISA Kit; Y-box-binding protein 1 ELISA Kit; YB 1 ELISA Kit; YB-1 ELISA Kit; YBOX1_HUMAN ELISA Kit; YBX 1 ELISA Kit; ybx1 ELISA Kit

缩写

YBX1

Uniprot No

P67809

灵敏度

7.8 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Epigenetics and Nuclear Signaling

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human YBX1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		89
		Range %		81-93
1:2		Average %		102
		Range %		95-109
1:4		Average %		95
		Range %		91-99
1:8		Average %		86
		Range %		83-91

回收率

The recovery of human YBX1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		104		101-109
EDTA plasma (n=4)		97		93-100

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected 2000 2.658 2.698 2.678 2.575 1000 2.381 2.451 2.416 2.313 500 1.974 1.962 1.968 1.865 250 1.229 1.169 1.199 1.096 125 0.698 0.687 0.693 0.590 62.5 0.400 0.412 0.406 0.303 31.25 0.209 0.211 0.210 0.107 0 0.101 0.105 0.103

货期

3-5 working days