产品描述
Introducing the Rat Xanthine Oxidase (XO) ELISA Kit - a highly sensitive and specific tool for the quantitative detection of Xanthine dehydrogenase in samples from Rattus norvegicus (Rat).
This kit is designed to measure Xanthine Oxidase (XO) levels in serum, plasma, and tissue homogenates with a detection range of 0.312 ng/mL to 20 ng/mL, and a sensitivity of 0.078 ng/mL. The assay principle is quantitative and uses a sandwich measurement method.
The assay is quick and easy, with an assay time of 1-5 hours and a sample volume of 50-100ul. The detection wavelength is 450 nm. The kit has been cited in more than 6 research papers and is a trusted and widely used product in the scientific community. Choose the Rat Xanthine Oxidase (XO) ELISA Kit for your research needs and get reliable and accurate results.
别名
Xdh ELISA Kit; Xanthine dehydrogenase/oxidase [Includes: Xanthine dehydrogenase ELISA Kit; XD ELISA Kit; EC 1.17.1.4); Xanthine oxidase ELISA Kit; XO ELISA Kit; EC 1.17.3.2 ELISA Kit; Xanthine oxidoreductase ELISA Kit; XOR)] ELISA Kit
缩写
XDH
Uniprot No
P22985
灵敏度
0.078 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Others
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat XOD in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
87 |
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| Range % |
82-93 |
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| 1:2 |
Average % |
96 |
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| Range % |
91-103 |
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| 1:4 |
Average % |
98 |
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| Range % |
96-105 |
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| 1:8 |
Average % |
97 |
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| Range % |
92-102 |
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回收率
| The recovery of rat XOD spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
98 |
94-104 |
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| EDTA plasma (n=4) |
104 |
96-108 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 20 |
2.711 |
2.610 |
2.661 |
2.544 |
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| 10 |
2.208 |
2.107 |
2.158 |
2.041 |
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| 5 |
1.507 |
1.486 |
1.497 |
1.380 |
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| 2.5 |
0.909 |
0.898 |
0.904 |
0.787 |
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| 1.25 |
0.467 |
0.456 |
0.462 |
0.345 |
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| 0.625 |
0.280 |
0.291 |
0.286 |
0.169 |
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| 0.312 |
0.185 |
0.188 |
0.187 |
0.070 |
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| 0 |
0.116 |
0.117 |
0.117 |
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货期
3-5 working days
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