产品描述
This Human α-MSH ELISA Kit was designed for the quantitative measurement of Human α-MSH protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.
别名
alpha-melanocyte stimulating hormone, alpha-MSH
缩写
α-MSH
灵敏度
0.039 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Biochemicals
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human α-MSH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 95 | |
| Range % | 92-104 | |
| 1:2 | Average % | 95 | |
| Range % | 91-104 | |
| 1:4 | Average % | 89 | |
| Range % | 85-93 | |
| 1:8 | Average % | 94 | |
| Range % | 86-98 | |
回收率
| The recovery of human α-MSH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 92 | 89-95 | |
| EDTA plasma (n=4) | 94 | 89-97 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| ng/ml | OD1 | OD2 | Average | Corrected | | | 10 | 2.081 | 2.181 | 2.131 | 2.000 | | | 5 | 1.256 | 1.198 | 1.227 | 1.096 | | | 2.5 | 0.655 | 0.635 | 0.645 | 0.514 | | | 1.25 | 0.406 | 0.416 | 0.411 | 0.280 | | | 0.625 | 0.260 | 0.270 | 0.265 | 0.134 | | | 0.312 | 0.220 | 0.219 | 0.220 | 0.089 | | | 0.156 | 0.187 | 0.182 | 0.185 | 0.054 | | | 0 | 0.132 | 0.130 | 0.131 | | |
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货期
3-5 working days