产品描述
This Bovine β-OHB ELISA Kit was designed for the quantitative measurement of Bovine β-OHB protein in serum, plasma. It is a Competitive ELISA kit, its detection range is 50 nmol/mL-800 nmol/mL and the sensitivity is 9.5 nmol/mL.
别名
beta-Hydroxybutyric acid, beta-OHB
缩写
β-OHB
灵敏度
9.5 nmol/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Others
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<15% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<15% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of bovine β-OHB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
90 |
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| Range % |
88-95 |
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| 1:2 |
Average % |
96 |
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| Range % |
91-103 |
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| 1:4 |
Average % |
98 |
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| Range % |
92-104 |
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| 1:8 |
Average % |
95 |
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| Range % |
89-100 |
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回收率
| The recovery of bovine β-OHB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
98 |
90-103 |
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| EDTA plasma (n=4) |
95 |
90-100 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| nmol/ml |
OD1 |
OD2 |
Average |
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| 800 |
0.136 |
0.138 |
0.137 |
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| 400 |
0.309 |
0.306 |
0.308 |
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| 200 |
0.674 |
0.682 |
0.678 |
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| 100 |
1.470 |
1.475 |
1.473 |
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| 50 |
2.706 |
2.712 |
2.709 |
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货期
3-5 working days