产品描述
This Horse β-EP ELISA Kit was designed for the quantitative measurement of Horse β-EP protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 3.12 pg/mL-200 pg/mL and the sensitivity is 0.78 pg/mL.
别名
beta-Endorphin, beta-EP
缩写
β-EP
灵敏度
0.78 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cancer
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of horse β-EP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |
| | Sample | Serum(n=4) | | |
| 1:1 | Average % | 97 | | |
| Range % | 90-111 | | |
| 1:2 | Average % | 105 | | |
| Range % | 102-109 | | |
| 1:4 | Average % | 93 | | |
| Range % | 90-97 | | |
| 1:8 | Average % | 88 | | |
| Range % | 85-93 | | |
回收率
| The recovery of horse β-EP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
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| Sample Type | Average % Recovery | Range | | |
| Serum (n=5) | 103 | 99-105 | | |
| EDTA plasma (n=4) | 91 | 90-94 | | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | | 200 | 1.899 | 1.922 | 1.911 | 1.788 | | | | 100 | 1.554 | 1.514 | 1.534 | 1.411 | | | | 50 | 1.123 | 1.078 | 1.101 | 0.978 | | | | 25 | 0.615 | 0.623 | 0.619 | 0.496 | | | | 12.5 | 0.421 | 0.456 | 0.439 | 0.316 | | | | 6.25 | 0.277 | 0.266 | 0.272 | 0.149 | | | | 3.12 | 0.185 | 0.194 | 0.190 | 0.067 | | | | 0 | 0.126 | 0.120 | 0.123 | | | |
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货期
3-5 working days