产品描述
This Pig β-TG ELISA Kit was designed for the quantitative measurement of Pig β-TG protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 3.12 ng/mL-200 ng/mL and the sensitivity is 0.78 ng/mL.
别名
beta-Thromboglobulin, beta-TG
缩写
β-TG
灵敏度
0.78 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Others
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of pig β-TG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |
| | Sample | Serum(n=4) | | |
| 1:1 | Average % | 89 | | |
| Range % | 84-93 | | |
| 1:2 | Average % | 104 | | |
| Range % | 100-108 | | |
| 1:4 | Average % | 96 | | |
| Range % | 91-100 | | |
| 1:8 | Average % | 87 | | |
| Range % | 82-91 | | |
回收率
| The recovery of pig β-TG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
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| Sample Type | Average % Recovery | Range | | |
| Serum (n=5) | 90 | 85-94 | | |
| EDTA plasma (n=4) | 95 | 91-99 | | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
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| ng/ml | OD1 | OD2 | Average | Corrected | | | | 20 | 2.783 | 2.648 | 2.716 | 2.584 | | | | 10 | 1.622 | 1.705 | 1.664 | 1.532 | | | | 5 | 0.996 | 0.992 | 0.994 | 0.862 | | | | 2.5 | 0.677 | 0.681 | 0.679 | 0.547 | | | | 1.25 | 0.428 | 0.443 | 0.436 | 0.304 | | | | 0.625 | 0.314 | 0.326 | 0.320 | 0.188 | | | | 0.312 | 0.172 | 0.169 | 0.171 | 0.039 | | | | 0 | 0.135 | 0.128 | 0.132 | | | |
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货期
3-5 working days