产品描述
This Human β-EP ELISA Kit was designed for the quantitative measurement of Human β-EP protein in serum, urine, cerebrospinal fluid (CSF), saliva, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 62.5 pg/mL-4000 pg/mL and the sensitivity is 15.6 pg/mL.
别名
beta-Endorphin, beta-EP
缩写
β-EP
灵敏度
15.6 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cancer
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human β-EP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 86 | |
| Range % | 80-92 | |
| 1:2 | Average % | 99 | |
| Range % | 91-110 | |
| 1:4 | Average % | 97 | |
| Range % | 92-105 | |
| 1:8 | Average % | 93 | |
| Range % | 86-98 | |
回收率
| The recovery of human β-EP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 95 | 89-99 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 4000 | 2.537 | 2.568 | 2.553 | 2.443 | | | 2000 | 2.010 | 1.999 | 2.005 | 1.895 | | | 1000 | 1.600 | 1.532 | 1.566 | 1.456 | | | 500 | 1.032 | 1.122 | 1.077 | 0.967 | | | 250 | 0.567 | 0.600 | 0.584 | 0.474 | | | 125 | 0.391 | 0.410 | 0.401 | 0.291 | | | 62.5 | 0.242 | 0.254 | 0.248 | 0.138 | | | 0 | 0.109 | 0.111 | 0.110 | | |
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货期
3-5 working days