产品描述
This Rabbit β-TG ELISA Kit was designed for the quantitative measurement of Rabbit β-TG protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 12.5 ng/mL-800 ng/mL and the sensitivity is 3.12 ng/mL.
别名
beta-Thromboglobulin, beta-TG
缩写
β-TG
灵敏度
3.12 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Others
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | | |
| Inter-assay Precision (Precision between assays):CV%<10% | | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit β-TG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |
| | Sample | Serum(n=4) | | |
| 1:1 | Average % | 91 | | |
| Range % | 86-95 | | |
| 1:2 | Average % | 102 | | |
| Range % | 97-107 | | |
| 1:4 | Average % | 91 | | |
| Range % | 85-97 | | |
| 1:8 | Average % | 97 | | |
| Range % | 91-103 | | |
回收率
| The recovery of rabbit β-TG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
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| Sample Type | Average % Recovery | Range | | |
| Serum (n=5) | 95 | 89-98 | | |
| EDTA plasma (n=4) | 97 | 90-100 | | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
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| ng/ml | OD1 | OD2 | Average | Corrected | | | | 800 | 2.801 | 2.869 | 2.835 | 2.748 | | | | 400 | 2.484 | 2.398 | 2.441 | 2.354 | | | | 200 | 1.716 | 1.768 | 1.742 | 1.655 | | | | 100 | 0.944 | 0.968 | 0.956 | 0.869 | | | | 50 | 0.454 | 0.468 | 0.461 | 0.374 | | | | 25 | 0.256 | 0.248 | 0.252 | 0.165 | | | | 12.5 | 0.147 | 0.151 | 0.149 | 0.062 | | | | 0 | 0.085 | 0.089 | 0.087 | | | |
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货期
3-5 working days