产品描述
TNF (肿瘤坏死因子, Tumor necrosis factor) 是由单核细胞、巨噬细胞和T淋巴细胞产生的细胞因子,是免疫系统中的重要组成部分。当机体免疫系统遭遇感染、外伤、缺血等刺激时,肿瘤坏死因子会被大量释放,发挥促炎症、促凋亡、抗肿瘤等生物学效应。肿瘤坏死因子对细胞的作用是双向调节的,过度的肿瘤坏死因子可能引发炎症反应甚至休克。在许多炎症、自身免疫病、肿瘤等疾病的发病机制中都扮演着重要角色。
华美生物所提供的Mouse Tumor necrosis factor α,TNF-α ELISA KIT属于ELISA检测试剂盒,采用双抗夹心法定量检测鼠血清、血浆、细胞培养上清、组织匀浆样本中的TNF,其灵敏度为3.9 pg/mL,检测范围为15.6 pg/mL-1000 pg/mL。
别名
Tnf ELISA Kit; Tnfa ELISA Kit; Tnfsf2 ELISA Kit; Tumor necrosis factor ELISA Kit; Cachectin ELISA Kit; TNF-alpha ELISA Kit; Tumor necrosis factor ligand superfamily member 2 ELISA Kit; TNF-a) [Cleaved into: Tumor necrosis factor ELISA Kit; membrane form ELISA Kit; N-terminal fragment ELISA Kit; NTF); Intracellular domain 1 ELISA Kit; ICD1); Intracellular domain 2 ELISA Kit; ICD2); C-domain 1; C-domain 2; Tumor necrosis factor ELISA Kit; soluble form] ELISA Kit
缩写
TNF
Uniprot No
P06804
灵敏度
3.9 pg/ml
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
|
| Three samples of known concentration were tested twenty times on one plate to assess. |
| Inter-assay Precision (Precision between assays): CV%<10% |
|
| Three samples of known concentration were tested in twenty assays to assess. |
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse TNF-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| |
| |
Sample |
Serum(n=4) |
|
| 1:1 |
Average % |
86 |
|
| |
Range % |
80-92 |
|
| 1:2 |
Average % |
95 |
|
| |
Range % |
91-105 |
|
| 1:4 |
Average % |
100 |
|
| |
Range % |
92-110 |
|
| 1:8 |
Average % |
92 |
|
| |
Range % |
86-98 |
|
回收率
| The recovery of mouse TNF-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
93 |
89-98 |
|
| EDTA plasma (n=4) |
95 |
90-100 |
|
标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
 |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 1000 |
2.793 |
2.822 |
2.808 |
2.651 |
|
| 500 |
1.889 |
1.943 |
1.916 |
1.759 |
|
| 250 |
1.245 |
1.211 |
1.228 |
1.071 |
|
| 125 |
0.729 |
0.686 |
0.708 |
0.551 |
|
| 62.5 |
0.373 |
0.404 |
0.388 |
0.232 |
|
| 31.2 |
0.287 |
0.298 |
0.292 |
0.136 |
|
| 15.6 |
0.231 |
0.244 |
0.238 |
0.081 |
|
| 0 |
0.152 |
0.161 |
0.157 |
|
|
货期
3-5 working days