产品描述
SCFR (干细胞因子受体) 是一种跨膜受体蛋白,它通过与干细胞因子的结合来调节干细胞的增殖、分化和生存。SCFR 的表达广泛分布于人体多种组织和细胞类型中,包括造血干细胞、成熟骨髓细胞、上皮细胞等。SCFR 在干细胞的自我更新和再生过程中发挥重要作用。
华美生物所提供的Human Stem Cell Factor Receptor,SCFR ELISA kit属于ELISA检测试剂盒,采用双抗夹心法定量检测人血清、血浆、组织匀浆样本中的SCFR,其灵敏度为0.39 ng/mL,检测范围为1.56 ng/mL-100 ng/mL。
别名
C Kit ELISA Kit; c-Kit ELISA Kit; c-Kit Ligand ELISA Kit; CD117 ELISA Kit; Kit ELISA Kit; Kit Ligand ELISA Kit; KIT oncogene ELISA Kit; KIT proto oncogene receptor tyrosine kinase ELISA Kit; KIT_HUMAN ELISA Kit; Mast cell growth factor receptor ELISA Kit; Mast/stem cell growth factor receptor Kit ELISA Kit; MGF ELISA Kit; p145 c-kit ELISA Kit; PBT ELISA Kit; Piebald trait protein ELISA Kit; Proto oncogene c Kit ELISA Kit; Proto oncogene tyrosine protein kinase Kit ELISA Kit; Proto-oncogene c-Kit ELISA Kit; SCF Receptor ELISA Kit; SCFR ELISA Kit; soluble KIT variant 1 ELISA Kit; Steel Factor Receptor ELISA Kit; Stem cell factor receptor ELISA Kit; tyrosine protein kinase Kit ELISA Kit; Tyrosine-protein kinase Kit ELISA Kit; v kit Hardy Zuckerman 4 feline sarcoma viral oncogene homolog ELISA Kit; v kit Hardy Zuckerman 4 feline sarcoma viral oncogene like protein ELISA Kit; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog ELISA Kit
缩写
SCFR
Uniprot No
P10721
灵敏度
0.39 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Others
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human SCFR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
93 |
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| Range % |
85-103 |
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| 1:2 |
Average % |
89 |
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| Range % |
85-99 |
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| 1:4 |
Average % |
93 |
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| Range % |
86-98 |
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| 1:8 |
Average % |
92 |
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| Range % |
83-100 |
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回收率
| The recovery of human SCFR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
93 |
86-101 |
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| EDTA plasma (n=4) |
97 |
91-105 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 100 |
1.891 |
1.916 |
1.904 |
1.741 |
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| 50 |
1.349 |
1.397 |
1.373 |
1.210 |
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| 25 |
0.954 |
0.992 |
0.973 |
0.810 |
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| 12.5 |
0.632 |
0.654 |
0.643 |
0.480 |
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| 6.25 |
0.435 |
0.426 |
0.431 |
0.268 |
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| 3.12 |
0.318 |
0.307 |
0.313 |
0.150 |
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| 1.56 |
0.249 |
0.254 |
0.252 |
0.089 |
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| 0 |
0.161 |
0.165 |
0.163 |
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货期
3-5 working days