产品描述
TNF(肿瘤坏死因子)是一种重要的细胞因子,在炎症反应和免疫系统调节中发挥着重要作用。TNF可由多种细胞产生,包括T细胞、单核细胞和巨噬细胞等。它能促进白细胞吞噬细菌,增加炎症反应中的细胞黏附分子表达,并在免疫应答中引导细胞凋亡。
华美生物所提供的Human Tumor necrosis factor α,TNF-α ELISA KIT属于ELISA检测试剂盒,采用双抗夹心法定量检测细胞培养上清样本中的TNF,其灵敏度为1.95 pg/mL,检测范围为7.8 pg/mL-500 pg/mL。
别名
APC1 ELISA Kit; APC1 protein ELISA Kit; Cachectin ELISA Kit; DIF ELISA Kit; Differentiation inducing factor ELISA Kit; Macrophage cytotoxic factor ELISA Kit; Tnf ELISA Kit; TNF superfamily member 2 ELISA Kit; TNF superfamily, member 2 ELISA Kit; TNF, macrophage derived ELISA Kit; TNF, monocyte derived ELISA Kit; TNF-a ELISA Kit; TNF-alpha ELISA Kit; TNFA ELISA Kit; TNFA_HUMAN ELISA Kit; TNFSF2 ELISA Kit; Tumor necrosis factor (TNF superfamily member 2) ELISA Kit; Tumor necrosis factor alpha ELISA Kit; Tumor necrosis factor ELISA Kit; Tumor necrosis factor ligand superfamily member 2 ELISA Kit; Tumor Necrosis Factor, Membrane Form ELISA Kit; Tumor necrosis factor, soluble form ELISA Kit
缩写
TNF
Uniprot No
P01375
灵敏度
1.95 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of humanTNF-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| |
| |
Sample |
Cell culture supernates(n=4) |
| 1:1 |
Average % |
90 |
| |
Range % |
86-94 |
| 1:2 |
Average % |
92 |
| |
Range % |
89-97 |
| 1:4 |
Average % |
95 |
| |
Range % |
91-99 |
| 1:8 |
Average % |
93 |
| |
Range % |
86-102 |
回收率
| The recovery of humanTNF-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| |
| Sample Type |
Average % Recovery |
Range |
| Cell culture supernates(n=5) |
98 |
94-103 |
标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |

| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
| 500 |
2.986 |
2.875 |
2.931 |
2.761 |
| 250 |
2.234 |
2.199 |
2.217 |
2.047 |
| 125 |
1.457 |
1.408 |
1.433 |
1.263 |
| 62.5 |
0.982 |
0.974 |
0.978 |
0.808 |
| 31.2 |
0.579 |
0.605 |
0.592 |
0.422 |
| 15.6 |
0.365 |
0.393 |
0.379 |
0.209 |
| 7.8 |
0.277 |
0.289 |
0.283 |
0.113 |
| 0 |
0.171 |
0.168 |
0.170 |
|
|
货期
3-5 working days