产品描述

This Human TNXB ELISA Kit was designed for the quantitative measurement of Human TNXB protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 46.88 ng/mL-3000 ng/mL and the sensitivity is 11.7 ng/mL.

别名

EDS3 ELISA Kit; Ehlers Danlos like syndrome ELISA Kit; Growth inhibiting protein 45 ELISA Kit; Hexabrachion like protein ELISA Kit; Hexabrachion-like protein ELISA Kit; HXBL ELISA Kit; NXB2 ELISA Kit; Tenascin X ELISA Kit; Tenascin X precursor ELISA Kit; Tenascin XB ELISA Kit; Tenascin XB1 ELISA Kit; Tenascin XB2 ELISA Kit; Tenascin-X ELISA Kit; TENX ELISA Kit; TENX_HUMAN ELISA Kit; TN X ELISA Kit; TN-X ELISA Kit; TNX ELISA Kit; TNXB ELISA Kit; TNXB1 ELISA Kit; TNXB2 ELISA Kit; TNXBS ELISA Kit; VUR8 ELISA Kit; XB ELISA Kit; XBS ELISA Kit

缩写

TNXB

Uniprot No

P22105

灵敏度

11.7 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Signal Transduction

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human TNXB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		93
		Range %		85-97
1:2		Average %		101
		Range %		95-105
1:4		Average %		92
		Range %		84-97
1:8		Average %		100
		Range %		92-105

回收率

The recovery of human TNXB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		92		85-97
EDTA plasma (n=4)		94		88-99

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected 3000 1.991 1.934 1.963 1.816 1500 1.609 1.576 1.593 1.446 750 1.230 1.200 1.215 1.068 375 0.732 0.712 0.722 0.575 187.5 0.530 0.507 0.519 0.372 93.75 0.369 0.355 0.362 0.215 46.88 0.261 0.257 0.259 0.112 0 0.149 0.145 0.147

货期

3-5 working days