产品描述
This Mouse PRF1 ELISA Kit was designed for the quantitative measurement of Mouse PRF1 protein in serum, plasma, cell culture supernates. It is a Sandwich ELISA kit, its detection range is 7.8 pg/mL-500 pg/mL and the sensitivity is 1.95 pg/mL.
别名
Prf1 ELISA Kit; Pfp ELISA Kit; Perforin-1 ELISA Kit; P1 ELISA Kit; Cytolysin ELISA Kit; Lymphocyte pore-forming protein ELISA Kit
缩写
PRF1
Uniprot No
P10820
灵敏度
1.95 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse PF/PFP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:20 | Average % | 98 | |
| Range % | 93-105 | |
| 1:40 | Average % | 93 | |
| Range % | 85-100 | |
| 1:80 | Average % | 86 | |
| Range % | 81-93 | |
| 1:160 | Average % | 93 | |
| Range % | 87-99 | |
回收率
| The recovery of mouse PF/PFP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 90 | 90-99 | |
| EDTA plasma (n=4) | 87 | 84-93 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 500 | 2.172 | 2.084 | 2.128 | 1.972 | | | 250 | 1.786 | 1.693 | 1.740 | 1.584 | | | 125 | 1.302 | 1.267 | 1.285 | 1.129 | | | 62.5 | 0.785 | 0.823 | 0.804 | 0.648 | | | 31.25 | 0.564 | 0.572 | 0.568 | 0.412 | | | 15.6 | 0.362 | 0.349 | 0.356 | 0.200 | | | 7.8 | 0.260 | 0.262 | 0.261 | 0.105 | | | 0 | 0.157 | 0.154 | 0.156 | | |
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货期
3-5 working days